Cytomark are dedicated to the development of cell stabilization reagents for use in Flow Cytometry

Blood collection Tubes:

Blood Collection

TransFix/EDTA Vacuum Blood Collection Tubes: stabilization of 3ml or 9ml of blood for up to 14 days

TransFix/EDTA Vacuum Blood Collection Tubes (TVTs) are plastic, direct draw collection tubes used to stabilize venous blood at the point of collection and preserve whole blood specimens for immunophenotyping by flow cytometry. TVTs are prefilled with sufficient TransFix containing K3EDTA for the immediate anticoagulation and stabilization of 3ml or 9ml of blood for up to 14 days.

Features of TVTs

Preserve the cell surface antigens of lymphocyte subsets for up to 14 days.
Prefilled with sufficient TransFix and K3EDTA to stabilize and anticoagulate a final draw volume of 3ml or 9ml of blood.
Multiple pack sizes available.
Immediate stabilization providing optimal sample stability.
Ease of application: TransFix/EDTA Vacuum Blood Collection Tubes fit docking sheaths from most manufacturers.
Sterilized by gamma radiation.

Benefits of TVTs

Reduced variability:
Ensures sample integrity during transportation between testing sites.
Reduction in pre-analysis variability – all samples preserved at the same time point.
Reduces the impact of unexpected delays in analysis
Convenience:
Easy to use – directly collect blood into TransFix then just mix by inversion.
Minimal “hands-on” time at collection sites
Reduced cost:
Greater efficiency in testing – allows for batching of samples prior to testing.
Prevent the need for repeat phlebotomy due to degraded samples.
Further tests can be performed on the same sample after the initial analysis, without subject recall.

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Ordering Information

TVT-03-01	3ml blood collection vacuum tube
TVT-03-02	2 pack of 3ml blood collection vacuum tubes
TVT-03-50	50 pack of 3ml blood collection vacuum tubes
TVT-09-01	9ml blood collection vacuum tube
TVT-09-02	2 pack of 9ml blood collection vacuum tubes
TVT-09-50	50 pack of 9ml blood collection vacuum tubes

Cerebrospinal Fluid Tubes (CSF):

Cerebrospinal Fluid (CSF)

TransFix/EDTA CSF Sample Storage Tubes contain TransFix that has been optimized for stabilizing key CSF leucocyte markers for up to 72 hours

Analysis of white blood cells in CSF is important in the diagnosis of many disorders. Flow cytometric analysis can provide absolute cell counts and detect rare events with high sensitivity and specificity. Cells within CSF samples are typically low in number, sparse in concentration, degrade rapidly ex vivo and only obtained via lumbar puncture.
TransFix/EDTA CSF Sample Storage Tubes contain TransFix that has been optimized for stabilizing key CSF leucocyte markers for up to 72 hours.

Features of CSF Tubes

The cellular component of CSF is stabilized for up to 72 hours allowing for determination of cell sub-populations and follow up analysis.
Higher lymphocyte yield after 30 minutes compared to untreated samples.
Allows gating of light scatter and key antigen expression patterns for up to 72 hours.
Quality controlled against EuroFlow SST Panel using CSF substitute, including the markers CD14, CD4, CD8, CD19, CD20, Igκ, Igλ, CD56. See Certificate of Conformity for full details.
Published data shows markers stabilized include CD10, CD19, CD34, CD45, HLA-DR, and CD117.
Recommended in guidelines published in the British Journal of Hematology.
Specific protocol for CSF Tubes with TransFix (see IFU).

Benefits of CSF Tubes

Reduced need for repeat lumbar puncture as a result of cell lysis:
Reduced costs
Reduced trauma for patients
Reduced time spent re-sampling.
Easy to use – just add CSF and mix by inversion
Ensures more time in which to conduct testing:
Eliminates the need for weekend and evening work
Allows for further investigation after initial cytomorphological analysis on the same sample
Reduces the impact of unexpected machine breakdown or staff shortages

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Ordering Information

TF-CSF-5-2	2 pack of 5ml CSF sample storage tubes
TF-CSF-5-10	10 pack of 5ml CSF sample storage tubes
TF-CSF-5-25	25 pack of 5ml CSF sample storage tubes
TF-CSF-5-50	50 pack of 5ml CSF sample storage tubes

Circulating Tumour Cells (CTC-TVTs):

Circulating Tumor Cells

Stabilization of CTCs for up to 5 days immediately preventing the degradation of CTCs and CTC antigens

TransFix stabilised blood is compatible with a range of methods for the isolation and analysis of circulating tumour cells (CTC). The number of CTC populations present in patient blood is very low, therefore the usefulness of CTC assessments depends upon accurate cell counts and the corresponding analysis of molecular targets. Circulating Tumour Cell TransFix/EDTA Vacuum Blood Collection Tubes (CTC-TVTs) are 9ml evacuated tubes prefilled with TransFix. CTC-TVTs are specifically designed for the optimal collection and stabilization of CTCs in whole human blood. They allow stabilization of CTCs for up to 5 days immediately preventing the degradation of CTCs and CTC antigens, allowing time to isolate and analyze these rare cells.

Benefits of CTC-TVTs

Easy to use – just collect blood and mix by inversion
Improved cell stability facilitating recovery of rare cells
Immediate Stabilization at the time point of venepuncture
Published examples show compatibility with a range of CTC isolation and analysis techniques:
Size exclusion filtration and by cellular surface markers
Microscopic analysis and enumeration of CTCs in metastatic breast and lung cancer patients
Analysis of CTCs through high-resolution image flow cytometry
Cell sorting and mutation analysis
mRNA extraction

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Ordering Information

CTC-TVT-09-02	2 pack of 9ml CTC Vacuum Tubes
CTC-TVT-09-50	50 pack of 9ml CTC Vacuum Tubes

TransFix Sample Storage Tubes (SST):

Sample Storage Tubes

TransFix is a cellular antigen stabilization solution that prevents cellular degradation in a variety of specimen types for flow cytometric analysis

TransFix is a cellular antigen stabilisation solution that prevents cellular degradation in a variety of specimen types for flow cytometric analysis. TransFix Sample Storage Tubes (SST) are ideal for use where blood specimens have already been collected in EDTA direct collection tubes.
TransFix Sample Storage Tubes (SST) consist of 1.2ml tubes containing 0.2ml of TransFix. These tubes do not contain an anti-coagulant.

Benefits of TransFix SSTs

Easy to use – just add sample and mix by inversion
Flexibility when receiving samples already anti-coagulated
Ensures sample integrity during transportation between clinical sites
Eliminates the need for weekend and evening work
Reduces the impact of unexpected machine breakdown or staff shortages
Allows for repeat testing of samples without the need for patient recall and repeat sampling
Greater efficiency in testing – allows for batching of samples prior to testing
Reduction in costs associated with the above advantages

Download Instruction For Use (RUO-RUS)>>

Ordering Information

TF-01-2	2 pack of 1.2ml TransFix SST tubes
TF-01-10	10 pack of 1.2ml TransFix SST tubes
TF-01-25	25 pack of 1.2ml TransFix SST tubes
TF-01-50	50 pack of 1.2ml TransFix SST tubes

TransFix® A Cellular Antigen Stabilization Reagent:

Transfix® Bulk

Transfix® Bulk is intended for stabilizing human whole blood specimens for immunophenotyping of white blood cells by flow cytometry

Transfix® Bulk is intended for stabilizing human whole blood specimens for immunophenotyping of white blood cells by flow cytometry. It is available as a CE/IVD and US GPR product, and can be purchased in 20ml or 1ml containers.
TransFix® can also be used for a variety of specimen types including: cerebrospinal fluid, lymph nodes, bone marrow, animal blood, circulating tumour cells, cell free RNA and exosome isolation.

Benefits of TransFix®

Stabilizes cell surface antigens and prevents cellular degradation for up to 14 days
Easy to use – just add TransFix® and mix by inversion
Ensures sample integrity during transportation between clinical sites
Eliminates the need for weekend and evening work
Reduces the impact of unexpected machine breakdown or staff shortages
Allows for repeat testing of samples without the need for patient recall and repeat sampling
Greater efficiency in testing – allows for batching of samples prior to testing
Reduction in costs associated with the above advantages

Download Instruction For Use (RUO-RUS)>>

Ordering Information

TFB-01-1	1ml TransFix
TFB-01-10	10 pack of 1ml TransFix
TFB-01-50	50 pack of 1ml TransFix
TFB-20-01	20ml TransFix

Quicklysis™ an erythrocyte lyse-non-wash solution:

Quicklysis™ (RUO)
by Cytognos

an erythrocyte lyse-non-wash solution without fixatives that allows studying leukocyte subpopulations by flow cytometry

Quicklysis™ is used in whole blood or bone marrow samples after immunofluorescence staining of the cells, and requires no subsequent washing-centrifugation steps reducing processing time and sample handling.

Benefits of Quicklysis™

The use of Quicklysis™ avoids cell losses associated to fixation and washing steps maintaining the relative distribution of sample cellular subsets and it is especially useful for absolute counts of CD34+ progenitor cell studies.
Lyse-non-wash approach is recommended for use when samples are treated with TransFix® from Cytomark

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Ordering Information

Catalog code	CYT-QL-1-R
Status	RUO
Target	Liquid lysis solution
Size	250 tests